7/8, 13, & 15
Professor Carl Spirito (Spirit o) / 1st of 5 professors to teach us
Goal is to teach basic mechanisms R/T principles of chem./physics
We are responsible for Guyton topics not covered in class, so use lecture as a guide to whats important and use lecture as a supplement to the text
LIBRARY reserve copies of old exams
He will give us handouts of overhead material
Substances move across cell membrane (from 1 compartment to another) in aqueous medium through
via: diffusion- solutes move from an area of inc conc to an area of lower conc, until eventually a uniform distribution of particles occurs. NET movement is what is considered. The solute particles move in random directions in straight lines, by simple molecular motion. Velocity determined by T, molec wt
Molecules bounce off wall and keep moving.
Probabilityafter particles move left to right then some will move right to left and eventually they will move in both directions. This is representative of a dynamic stable equilibrium(no further net movt occurring)
A static equilibrium is where no movement occurs at all, which theoretically occurs at 0K
Diffusion rates vary according to size
diffusion works great over tiny distances but is inefficient over large areas, such as in multicellular animals. This is why resp, CV, renal and other systems evolved so substrates could move to needed areas over large distances.
Ficks law describes diffusion in terms of proportionality- and looks at proportionality of movement in one direction as well as movement in another direction. It considers back flow which is considered in NET movement
Flux/flow prop to T, x sec area, conc gradient, 1/chg x these are conditions
of the solution; and to 1/molecular weight which is related to molecule size
inc T = inc motion
larger particles move slower than small ones
Now add a membrane with slits, like a lipid bilayer membrane
Diffusion is affected by adding a lipid membrane and listed below are the things which determine--- molecule passage through the membrane
Mechanisms in cell membrane evolved to allow for polar molecules like proteins, AA, ions,
See hand outs they are very helpful
Channels for diffusion PASSIVE protein particles move via simple diffusion
Water and u shaped tube drawing water will move from an area of high conc (no solute) to an area of less conc (solute mixed with water), thus causing a shift in the once even fluid level on both sides of the u ( as long as membrane does not let particles thru just water). Think of how gravity affects the level of the u where the water and solute are. There are 2 opposing forces
Osmosis deals with diffusion of water
Tonicity- deals with what solution is
When thinking of tonicity, visualize water as the solute being dissolved, and the concepts of tonicity will be more clear.
Metabolic energy to push substance against conc gradient
- carrier protein forms complex with substrate to be carried and complex
and which conformational change ΰ substrate moves through cell
where the conc grad of itself is high and release is not automatic
supply energy via a strong phosphate bond dissociation, and substrate is
Mechanisms to maintain cell equilibrium
concentration gradient utilized to do movement, which itself was derived from ATP process.
Think of concentration gradient in A&B , in cells and how substrate moves thru to each area and which mechanisms enable substrate to move in
Think of levels of substrate in each compartment and its relationship to its concentration gradient.
Body fluid compartments - ICF - RBC=2L + 25L
ECF- plasma = 3L + 11LHow does food get from GI to plasma ECF & ICF
Fluid out via kidney renal tubule & plasma
A little water is synthesized from oxidation but most is obtained through diet and is lost through feces, sweat, exhalation. EQUILIBRIUM is evident by seeing 3L in and 3L out
Measuring fluid volume - via appropriately sized tracers ( about 1/10 mole) and circulate them through the body compartments via cell membrane (they must be able to fit through the pores). Now take blood sample and measure cpmc of tracers present. Looking for volume so moles of tracer / ml or L
When measuring ECF be sure to choose a molecule that will fit into a capillary but not into the cell.
Plasma moderate protein 90%
ECF high levels of Na, Cl,
low K, contains 60% lower protein(1/4)that of plasma
Is a virtual space where a matrix exists its not sloshy like plasma, and its
A proteoglypex, has collagen, not alot of volume, its a pathway or a link from what happens in cells and plasma
ICF- high K, Ph, Mg
Low Na, Cl
Very high protein protein comprises 97% of mass of ICF, therefore it has the highest protein content of all 3 areas
Capillary flow can be conceptualized to understand substance and H2O flow across a membrane
Membrane, and there is no smooth muscle because its a passive tube
Forces in the system
When thinking about oncotic pressure think of it like that things that cant move are classified as the solute, and things that can move are classified as solvents-ions, small particles
Net movement means there is movement to one side of the cell membrane more than to the other. Measuring it can give the gross numbers
Ions move in RT concentration gradient of particle and charge
Osmotic pressure is a force = to the force causing water to move
Tonicity is a comparative measure X fluid compared to Y fluid. Plasma is generally referenced if no other fluid is mentioned.
In discussing ICF vs ECF, RBCs are a subdivision of ICS, and plasma is a subdivision of ECF
Fluid volume is measured with in the different spaces with tracer molecules , which will be restored to one compartment
Tracer X, the cell membrane determines total body volume is being measured, but if the tracer is constricted to the vascular space due to the intracellular clefts in the capillary, then only the vasc space can be measured.
The capillary wall is an example of fluid and solute movement. The single cell layer with clefts allow a diameter molecule the width of 200 Na atom to fit thru which is big. Egs are AA, water, small plasma proteins.
Hydrophobic water thru pores & thru cell wall sm. Charged ions, Na, Cl, K
Lipophilic Inc solubility resp gasses so there is no barrier to CO2 and O2
Diffusion is regulated by concentration difference of substances on both side of the cap wall and each ion has its own concentration gradient. Think it as a ratio 3 Na 1 K
1Na 3K see pic 1
Know the different forces diffusion, bulk, COP
Diffusion moves 10% of stuff
There is 50-75 microns across a cell, the net exchange occurs slowly unless the concentration gradient is vast
FORCE - Bulk flow moves 90% of the substances and water driven by diffusion, from hydrostatic pressure, from one area the other. Cap BP cap bp 10
The cap BP at the beginning is higher than the ECFs COP. Water, solutes, ions, AA, glucose, proteins are pushed there is no discrimination sas to what's pushed through the clefts as long the stuff fits through.
Contrast this to specific types of diffusion. AT, gated and carrier proteins
FORCE Oncotic pressure (COP) OP due to the presence of substances which dont pass through the wall. COP causes movement of substrate from ECF to capillary. Water and what ever else fits will move into the capillary. The solvent is thought of as any substance which is drawn to the protein, which could be water, glucose, Na ect
see pic 2
Think of what forces move what stuff in what direction.
There are 4 times as much proteins in the plasma than in the ECF which usually keeps water from building up in the ECF, but smaller proteins move out in the ECF and draw water to them but this excess water is removed by the lymphatic are returned to the vasc space.
See pic 3
plasma is considered to be continuous with the ECF, when comparing the non protein content of each plasma & ECF, because other than the protein the composition is the almost the same.
The lymph moves proteins to the thoracic duct which drains into the thoracic system
The net positive fluid dump into the circ system returns that fluid back to the caps, he change in volume is balanced by the lymph.
FORCES ARTERIAL VENOUS
CAP BEGINS CAP ENDS
Outward Forces (out of cap)
HYDROSTATIC PR 30mmHg 10mmHg
ONCOTIC PR 5 mmHg 5mmHg
ECF FLD PR 6mmHg 6mmHg
41 mmHg 21mmHg
Inward Forces (into cap)
ONCOTIC PR 28mmHg 21MMhg
So, 41-28=23mmHg the net oncotic force is 23 in, because there is > protein in the capillary than in the ECF, so water is drawn to the protein in the capillary.
21-28= a difference of 7, which shows a net inward force of 7
@ 90% of stuff moved out is reabsorbed and the rest 10% is what forms the lymphatic flow.
?????????????????? net force outward force at the arterial end of the cap is out 13, ar a + 13 net in force. Tendency is for the fluid to move out at arterial end and back in in the venous end.
Pic 5 at any given temp there is movement in both directions (of fluid)
Neg ECF fluid pr the physical pressure is negative in the ECF. There is very little fluid in there. Oncotic pressure moves water out of the capillary into the ECF.
99½% of plasma doesnt leave capillary during flowing through it, and ½ of 1 % of plasma moves out, mixes with ECF and moves back in. This system works over the long haul.
A typical capillary is 11mm long, and there is a limited for the exchange to occur. 1/200th of Na, K, water, etc actually leaves the capillary and contributes to the mixing, so the exchanges occur in tiny increments over continuous time.
Think of diffusion and conc gradient, and how the flow is between the capillary to the tissue and back to the cap again.
Edema bulk force Hydrostatic pressure of 30, and if BP is up, the blood flow will be inc and more fluid will be forced out of blood vessel into tissue. ΰ which inc fld in ECF. Cardiac problems, venous clots can cause an inc in hydrostatic pressures locally or systemically
Oncotic force suddenly dec protein in blood from say burns, dec nut, renal ds, and others can change the oncotic balance and < fluid enters caps and > fluid stays behind in the tissues, esp if the proteins leak into the ECF.
Inc pouricity fo intra cellular clefts of caps will cause larger proteins to enter ECF and this will change oncotic balance and fluid stays in tissue
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